High resolution profiling of cell cycle-dependent protein and phosphorylation abundance changes in non-transformed cells.

ABSTRACT: The cell cycle governs a precise series of molecular events, regulated by coordinated changes in protein and phosphorylation abundance, that culminates in the generation of two daughter cells. Here, we present a proteomic and phosphoproteomic analysis of the human cell cycle in hTERT-RPE-1 cells using deep quantitative mass spectrometry by isobaric labelling. By analysing non-transformed cells and improving the temporal resolution and coverage of key cell cycle regulators, we present a dataset of cell cycle-dependent protein and phosphorylation site oscillation that offers a foundational reference for investigating cell cycle regulation. These data reveal regulatory intricacies including proteins and phosphorylation sites exhibiting cell cycle-dependent oscillation, and proteins targeted f

SUBMITTER: Rega C 

PROVIDER: S-EPMC11910661 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature