Intracellular Mg²⁺ concentrations are differentially regulated in the sperm head and mid-piece in acrosome reaction inducing conditions.

ABSTRACT: The sperm ability to fertilize involves the regulation of ATP levels. Because inside cells, ATP is complexed with Mg²⁺ ions, changes in ATP levels result in changes in intracellular Mg²⁺ concentration ([Mg²⁺]_i), which can be followed using intracellular Mg²⁺ sensors such as Mag-520. In this work, we tested conditions known to decrease sperm ATP such as starvation and capacitation. As expected, in these conditions [Mg²⁺]_i increased in all cell compartments. In contrast, when ATP increases, such as adding nutrients to starved sperm, [Mg²⁺]_i significantly decreases in all compartments. On the other hand, when the acrosome reaction was induced, either with progesterone or with ionomycin, [Mg

SUBMITTER: Sanchez-Cardenas C 

PROVIDER: S-EPMC11910749 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature