Acetol biosynthesis enables NADPH balance during nitrogen limitation in engineered Escherichia coli.

ABSTRACT:

Background

Nutrient limitation strategies are commonly applied in bioprocess development to engineered microorganisms to further maximize the production of the target molecule towards theoretical limits. Biomass formation is often limited under the limitation of key nutrients, and understanding how fluxes in central carbon metabolism are re-routed during the transition from nutrient excess to nutrient-limited condition is vital to target and tailor metabolic engineering strategies. Here, we report the physiology and intracellular flux distribution of an engineered acetol-producing Escherichia coli on glycerol under nitrogen-limited, non-growing production conditions.

Results

Acetol production in the engineered E. coli strain is triggered upon nitrogen depletion. During nitro

SUBMITTER: Sudarsan S 

PROVIDER: S-EPMC11910842 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature