Single-cell transcriptomic analysis reveals dynamic changes in the liver microenvironment during colorectal cancer metastatic progression.

ABSTRACT:

Background

Metastasis is a leading cause of cancer-related deaths, with the liver being the most frequent site of metastasis in colorectal cancer. Previous studies have predominantly focused on the influence of the primary tumor itself on metastasis, with relatively limited research examining the changes within target organs.

Methods

Using an orthotopic mouse model of colorectal cancer, single-cell sequencing was employed to profile the transcriptomic landscape of pre-metastatic and metastatic livers. The analysis focused on identifying cellular and molecular changes within the hepatic microenvironment, with particular emphasis on inflammatory pathways and immune cell populations.

Results

A neutrophil subpopulation with high Prok2 expression was identified, showing el

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC11910851 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature