Validation of reliable reference genes for comparison of gene expression across species in the Anopheles Hyrcanus Group.

ABSTRACT: The Anopheles Hyrcanus Group comprises 25 recognized mosquito species that exhibit significant variations in critical vector characteristics despite having similar morphology and ecological niches. Cross-species transcriptional profiling with appropriate reference genes is essential to evaluate the physiological differences among these closely related species. This study aimed to identify reliable pan-species reference genes among six Anopheles Hyrcanus Group species found in South Korea. We assessed the expression stability of eight candidate genes across five developmental stages in each of the six species. Utilizing qPCR and analysis programs, our analysis revealed that the transcription of ribosomal protein L8 (RPL8) and ribosomal protein L13a was the most stable at the larval stage. I

SUBMITTER: Lee DE 

PROVIDER: S-EPMC11911449 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature