Repeat-dose toxicity of human umbilical cord mesenchymal stem cells via subcutaneous injection in NOG mice.

ABSTRACT:

Background

Stem cell therapy shows promise for treating skin diseases and enhancing medical aesthetics. However, safety data for subcutaneous injection of stem cells remain limited. In this study, we evaluated the toxicity of human umbilical cord mesenchymal stem cells (hUC-MSCs) in NOD. Cg-Prkdc^scidIL2rg^tm1Sug/JicCrl (NOG) mice.

Methods

Mice received subcutaneous hUC-MSC injections at doses of 2.5 × 10⁷ and 2.0 × 10⁸ cells/kg on days 1, 8, 12, 16, and 20, followed by withdrawal and observation for 6 weeks. Toxicity was assessed through clinical observation, behavioral analysis, pathology, organ weight measurements, and histopathology. hUC-MSC distribution was determined via validated quantitative (q)PCR and colonization was assess

SUBMITTER: Xia L 

PROVIDER: S-EPMC11911471 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature