Integration of GWAS and transcriptome analysis to identify temperature-dependent genes involved in germination of rapeseed (<i>Brassica napus</i> L.).

ABSTRACT: Low temperature germination (LTG) is one of crucial agronomic traits for field-grown rapeseed in the Yangtze River Basin, where delayed sowing frequently exposes germination to cold stress. Because of its importance, the genetic basis underlying rapeseed germination under different temperatures has been continuously focused. By long-term field observation, we screened out two cultivars with significantly different LTG performance (JY1621 and JY1605) in field and lab conditions, which therefore were further used for the transcriptome sequencings at three key timepoints under normal and low temperatures. Comparative analysis among multiple groups of differentially expressed genes (DEGs) revealed a set of either early or late temperature response germination (ETRG or LTRG) genes, as well as c

SUBMITTER: Wang R 

PROVIDER: S-EPMC11911475 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature