Real-time behavioral monitoring of C57BL/6J mice during reproductive cycle.

ABSTRACT:

Introduction

The present study aims to identify differences in behavioral profiles in post-pubertal C57BL/6J males and female mice across distinct phases of the reproductive cycle in a home cage environment.

Methods

To reduce human bias, we used an automated behavioral analysis system HomeCageScan from CleverSys Inc. Mice were monitored continuously, and resulting data were summarized across 24-h, light, and dark cycles. Behavioral activities of each period were analyzed using hierarchical clustering, factor analysis, and principal component analysis.

Results

Females exhibited higher levels of physically demanding activities, including ambulatory and exploratory movements, particularly during estrus and metestrus, with estrus showing up to 30% more activity tha

SUBMITTER: Khatiz A 

PROVIDER: S-EPMC11911481 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature