Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solani AG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum.

ABSTRACT: Rhizoctonia solani AG1 IA is a harmful necrotrophic fungus responsible for various crop diseases, including maize and rice sheath blight, which can lead to significant production losses. However, the pathogenic mechanisms and the roles of effectors in this pathogen remain poorly understood. In this study, we identified a glycoside hydrolase 16 family gene, RsEG146, from R. solani that was upregulated during its infection of Zea mays leaves. When transiently expressed through agroinfiltration, RsEG146 induced cell death in the leaves of tobacco (Nicotiana tabacum 'Samsun'). The predicted signal peptide of RsEG146 was essential for its cell death-inducing activity, while the conserved enzymic active site was not required. The chitin-binding domain was critical for the cell death-inducing act

SUBMITTER: Chen C 

PROVIDER: S-EPMC11911542 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature