Utilization of the QuPath open-source software platform for analysis of mammalian spermatogenesis†.

ABSTRACT: The adult mammalian testis is filled with seminiferous tubules, which contain somatic Sertoli cells along with germ cells undergoing all phases of spermatogenesis. During spermatogenesis in postnatal mice, male germ cells undergo at least 17 different nomenclature changes as they proceed through mitosis as spermatogonia (=8), meiosis as spermatocytes (=6), and spermiogenesis as spermatids (=3). Adding to this complexity, combinations of germ cells at each of these stages of development are clumped together along the length of the seminiferous tubules. Due to this, considerable expertise is required for investigators to accurately analyze changes in spermatogenesis in animals that have spontaneous mutations, have been genetically modified (transgenic or knockout/knockin), or have been treat

SUBMITTER: Niedenberger BA 

PROVIDER: S-EPMC11911557 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature