Orphan GPR50 Restrains Neurite Outgrowth and Cell Migration by Activating the G_12/13 Protein-RhoA Pathway in Neural Progenitor Cells and Tanycytes.

ABSTRACT: Human genetic variants of the orphan G protein-coupled receptor GPR50 are suggested risk factors for neuropsychiatric disorders. However, the function of GPR50 in the central nervous system (CNS) and its link to CNS disorders remain poorly defined. Here, we generated GPR50 knockout (GPR50-KO) mice and show that the absence of GPR50 increases neurite outgrowth, cell motility and migration of isolated neural progenitor cells (NPCs) and hypothalamic radial glial cells (tanycytes). These observations were phenocopied in NPCs and tanycytes from wild-type mice treated with neutralizing antibodies the against the prototypical neurite growth inhibitor Nogo-A. Treatment of NPCs and tanycytes from GPR50-KO cells with neutralizing antibodies had no further, additive, effect. Inhibition of neurite gro

SUBMITTER: Ahmad R 

PROVIDER: S-EPMC11911906 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature