Talin1 Ser425 phosphorylation promotes colorectal cancer progression and metastasis.

ABSTRACT:

Background

Talin1 serves as a crucial element within the multiprotein adhesion complexes that facilitate processes such as cell migration, adhesion, and integrin signaling. This study aimed to explore the underlying role of Talin1 Ser425 phosphorylation in the development of colorectal cancer (CRC).

Methods

Blank plasmids, non-phosphorylatable mutant Talin1 S425A plasmids, and phosphorylation-mimetic mutant Talin1 S425D plasmids were constructed and used for transfection of CRC cells. The expression of mRNA and protein in CRC cells or tumor tissues was assessed by The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and UALCAN databases, immunohistochemistry (IHC), and Western blot (WB). Cell proliferation was assessed via 5-ethynyl-2-deoxyuridine (EDU) prolife

SUBMITTER: He Z 

PROVIDER: S-EPMC11912083 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature