Dynamic Measurement of Protein Translation in Mycobacteria Using Nontargeted Stable Isotope Labeling in Combination with MALDI-TOF Mass Spectrometry-Based Readout.

ABSTRACT: Understanding the metabolic pathways of mycobacteria is essential to identify novel antibiotics and to compose synergistic antibiotic regimens against tuberculosis, one of the world's most deadly infectious diseases with >1.7 Mio yearly deaths. We present a novel proteomics approach for the dynamic measurement of the nascent fractions of specific proteins. We use nontargeted stable isotope incorporation to label the nascent proteins after adding glycerol-1,3-¹³C₂. The analysis is performed using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with a self-programmed script, allowing quantitative data. We compared the de novo synthesis of proteins under regular growth conditions and the effect of four antimicrobials, in

SUBMITTER: Haisch C 

PROVIDER: S-EPMC11912119 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature