One-Step Immunoassay for Biomarker Quantification in Complex Mixtures Based on Phase-Separated Antifouling Coacervates.

ABSTRACT: We develop and present a one-pot sandwich immunoassay (termed oneSTEP) to detect target biomolecules in complex biological fluids based on programmable zwitterionic polymer coacervates. We design these coacervates to selectively recruit target analytes with ultralow nonspecific adsorption. We show that dynamic compartmentalization combined with local target enrichment delivers a rapid and wash-free sandwich immunoassay with high specificity and a high signal-to-noise ratio. The fluorescence-based readout is performed using standard microscopy methods and flow cytometry. We demonstrate the capabilities of the oneSTEP assay by detecting complement component 5 in human serum and the spike protein of severe acute respiratory syndrome coronavirus 2 in artificial saliva with a limit of detection

SUBMITTER: Lenzen PS 

PROVIDER: S-EPMC11912134 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature