Enantiopurity by Directed Evolution of Crystal Stabilities and Nonequilibrium Crystallization.

ABSTRACT: Crystallization is a powerful method to isolate enantiopure molecules from racemates if enantiomers self-sort into separate enantiopure crystals. Unfortunately, this behavior is unpredictable and rare (5-10%), as both enantiomers predominantly crystallize together to form racemic crystals, hindering any such chiral sorting. These unfavorable statistics might be overcome using nonequilibrium conditions. Therefore, we systematically characterize energy differences (ΔG^Φ) between racemic and enantiopure crystal phases for libraries of target molecules (phenylglycine, praziquantel) with different chemical modifications. Surprisingly, these libraries reveal wide but similar continuous distributions of ΔG^Φ, wherein similar chemical modifications group together.

SUBMITTER: Pinetre C 

PROVIDER: S-EPMC11912332 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature