RNA-targeted proteomics identifies YBX1 as critical for efficient HCMV mRNA translation.

ABSTRACT: Viruses have evolved unique strategies to circumvent host control of protein synthesis and enable viral protein synthesis in the face of the host response. Defining the factors that regulate viral messenger RNA (mRNA) translation is thus critical to understand how viruses replicate and cause disease. To identify factors that might regulate viral mRNA translation, we developed a technique for identifying proteins associated with a native RNA expressed from its endogenous promoter and genomic locus. This approach uses a guide RNA to target dCas13b fused to a biotin ligase domain to a specific RNA, where it covalently labels proteins in close proximity. Using this approach, we identified multiple proteins associated with transcripts encoding the human cytomegalovirus (HCMV) IE1 and IE2 protei

SUBMITTER: Dickmander RJ 

PROVIDER: S-EPMC11912382 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature