Towards unraveling antimicrobial resistance dynamics: a longitudinal exploration of rectal swab metagenomes.

ABSTRACT: The increasing prevalence of antimicrobial resistance (AMR) poses significant challenges in clinical settings. In particular, early screening and detection of colonization by multidrug-resistant organisms (MDROs) in patients at admission is crucial. In this context, the clinical use of metagenomics (mNGS) holds promise for fast and untargeted diagnostic methods. Here, we aimed to evaluate the long-term stability of the rectal microbiome and the diagnostic accuracy of mNGS in comparison to culture and whole-genome sequencing (WGS) of MDROs. We analyzed rectal swabs from 26 patients with two consecutive admissions over a four-year period. The detected antimicrobial resistance genes and assembled metagenomes were compared to those obtained via classical culture-based antimicrobial susceptibil

SUBMITTER: Boutin S 

PROVIDER: S-EPMC11912604 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature