O8G-modified circPLCE1 inhibits lung cancer progression via chaperone-mediated autophagy.

ABSTRACT:

Background

Lung cancer poses a serious threat to human health, but its molecular mechanisms remain unclear. Circular RNAs (circRNAs) are closely associated with tumour progression, and the important role of 8-oxoguanine (o8G) modification in regulating the fate of RNA has been gradually revealed. However, o8G modification of circRNAs has not been reported. We identified circPLCE1, which is significantly downregulated in lung cancer, and further investigated the o8G modification of circPLCE1 and the related mechanism in lung cancer progression.

Methods

We identified differentially expressed circRNAs by RNA high-throughput sequencing and then conducted methylated RNA immunoprecipitation (MeRIP), immunofluorescence (IF) analysis, crosslinking immunoprecipitation (CLIP) and acti

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC11912650 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature