Characterization of pediatric urinary microbiome at species-level resolution indicates variation due to sex, age, and urologic history.

ABSTRACT:

Background

Recently, associations between recurrent urinary tract infections (UTI) and the urinary microbiome (urobiome) composition have been identified in adults. However, little is known about the urobiome in children. We aimed to characterize the urobiome of children with species-level resolution and to identify associations based on UTI history.

Study design

Fifty-four children (31 females and 21 males) from 3 months to 11 years of age participated in the study. Catheterized urine specimens were obtained from children undergoing a clinically indicated voiding cystourethrogram. To improve the analysis of the pediatric urobiome, we used a novel protocol using filters to collect biomass from the urine coupled with synthetic long-read 16S rRNA gene sequencing to obtain cult

SUBMITTER: Kelly MS 

PROVIDER: S-EPMC11913066 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature