Selective Activation of Peptide-Thioester Precursors for Templated Native Chemical Ligations.

ABSTRACT: Chemical protein synthesis enables access to proteins that would otherwise be difficult or impossible to obtain with traditional means such as recombinant expression. Chemoselective ligations provide the ability to join peptide segments prepared by solid-phase peptide synthesis. While native chemical ligation (NCL) is widely used, it is limited by the need for C-terminal thioesters with suitable reaction kinetics, properly placed native Cys or thiolated derivatives, and peptide segment solubility at low mM concentrations. Moreover, repetitive purifications to isolate ligated products are often yield-sapping, hampering efficiency and progress. In this work, we demonstrate the use of Controlled Activation of Peptides for Templated NCL (CAPTN). This traceless multi-segment templated NCL appro

SUBMITTER: Spaltenstein P 

PROVIDER: S-EPMC11913120 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature