Specific enhancement of the translation of thermospermine-responsive uORF-containing mRNAs by ribosomal mutations in <i>Arabidopsis thaliana</i>.

ABSTRACT: Auxin-induced xylem formation in angiosperms is negatively regulated by thermospermine, whose biosynthesis is also induced by auxin. In Arabidopsis thaliana, loss-of-function mutants of ACL5, which encodes thermospermine synthase, exhibit a dwarf phenotype accompanied by excessive xylem formation. Studies of suppressor mutants that recover from the acl5 dwarf phenotype suggest that thermospermine alleviates the inhibitory effect of an upstream open-reading frame (uORF) on the main ORF translation of SAC51 mRNA. Many suppressor mutations for acl5 have been mapped to the uORF conserved in the SAC51 family or to ribosomal protein genes, such as RPL10A, RPL4A, and RACK1A. In this study, we identified newly isolated acl5 supp

SUBMITTER: Mutsuda K 

PROVIDER: S-EPMC11913374 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature