Noncoding function of super enhancer derived <i>Cpox</i> pre-mRNA in modulating neighbouring gene expression and chromatin interactions.

ABSTRACT: Super enhancers are important regulators of gene expression that often overlap with protein-coding genes. However, it is unclear whether the overlapping protein-coding genes and the RNA derived from them contribute to enhancer activity. Using an erythroid-specific super enhancer that overlaps the Cpox gene as a model, Cpox pre-mRNA is found to have a non-coding function in regulating neighbouring protein-coding genes, eRNA expression and TAD interactions. Depletion of Cpox pre-mRNA leads to accumulation of H3K27me3 and release of p300 from the Cpox locus, activating an intra-TAD enhancer and gene expression. Additionally, a head-to-tail interaction between the TAD boundary genes Cpox and Dcbld2 is identified, facilitated by a novel type of repressi

SUBMITTER: Xie B 

PROVIDER: S-EPMC11913378 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature