Novel insights into carbohydrate utilisation, antimicrobial resistance, and sporulation potential in <i>Roseburia intestinalis</i> isolates across diverse geographical locations.

ABSTRACT: Roseburia intestinalis is one of the most abundant and important butyrate-producing human gut anaerobic bacteria that plays an important role in maintaining health and is a potential next-generation probiotic. We investigated the pangenome of 16 distinct strains, isolated over several decades, identifying local and time-specific adaptations. More than 50% of the genes in each individual strain were assigned to the core genome, and 77% of the cloud genes were unique to individual strains, revealing the high level of genome conservation. Co-carriage of the same enzymes involved in carbohydrate binding and degradation in all strains highlighted major pathways in carbohydrate utilization and reveal the importance of xylan, starch and mannose as key growth substrates. A single strain had

SUBMITTER: Mukhopadhya I 

PROVIDER: S-EPMC11913394 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature