Single-cell RNA sequencing of peripheral blood mononuclear cells from bronchopulmonary dysplasia.

ABSTRACT:

Background

Bronchopulmonary dysplasia (BPD) is a severe respiratory disease that primarily affects premature infants, characterized by persistent inflammation and abnormal immune activation. This study aimed to elucidate the immunological mechanisms underlying BPD by integrating single-cell RNA sequencing with T/B cell receptor profiling of peripheral blood mononuclear cells (PBMCs) from preterm infants with BPD, complemented by validation in a murine BPD model.

Methods

We profiled PBMCs from preterm infants diagnosed with BPD and healthy controls, identifying 22 distinct cell clusters corresponding to major immune cell types.

Results

Significant alterations were observed in myeloid and lymphoid subsets, with neutrophils undergoing metabolic reprogramming toward oxida

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11913593 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature