Luteolin Inhibits Dexamethasone-Induced Osteoporosis by Autophagy Activation Through miR-125b-5p/SIRT3/AMPK/mTOR Axis, an In Vitro and In Vivo Study.

ABSTRACT: Luteolin (LUT) has been suggested as an inhibitor of osteoporosis (OP). This investigation examines the pivotal role of the miR-125b-5p/SIRT3/AMPK/mTOR pathway in mediating luteolin-induced effects on OP. Mesenchymal stem cells derived from bone marrow (BMSCs) were exposed to dexamethasone (DEX) to create an in vitro model of OP. Following treatment with luteolin, the levels of miR-125b-5p and SIRT3 were quantified using reverse transcription polymerase chain reaction. Moreover, SIRT3, AMPK, mTOR protein levels, and osteogenesis (OPN, Runx2, OSX, and OCN), and autophagy (p62, ATG5, LC3, and BECN1) were evaluated using ELISA. Additionally, specific mimics and siRNA were constructed to overexpress miR-125b-5p or downregulate SIRT3. Furthermore, animal models of DEX-induced OP were constructe

SUBMITTER: Tang L 

PROVIDER: S-EPMC11913733 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature