INS2 lineage cell tracking and insulin expression in the related organs of mice.

ABSTRACT: We tracked lineage map of the Ins2 cells in mice and insulin expression in migration sites of cells. We studied effect of the Wnt/β-catenin signaling pathway on the migration. We studied insulin secretion status in submandibular gland of mice under hyperglycemia stress. Cre/loxp system was used to observe migration sites and timing of the Ins2-cre lineage cells. Immunohistochemistry and immunofluorescence was used to detecte presence of insulin in the Ins2-cre lineage cells. Knockout mice from E9.5 to adulthood was studied to explore role of the Wnt/β-catenin on the migration. Immunofluorescence and the QRT-PCR (Quantitative Real-time Polymerase Chain Reaction) was used to study insulin secretion in submandibular gland under hyperglycemic conditions. Expression sites of the Ins2-cre gene i

SUBMITTER: Xiang KZ 

PROVIDER: S-EPMC11914036 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature