A novel simplified structural design as an artificial enzyme for efficient hydrolysis of PNPA.

ABSTRACT: In this study, a novel artificial enzyme Zn(II)-SMM with simple organic structure for PNPA-directed hydrolysis is proposed. The introduction of hydrophobic quinoline and dipyridinium in Zn(II)-SMM system demonstrates high activity for the complexation of Zn²⁺ and hydrolysis of PNPA, and the catalytic rate (k_cat/k_uncat) exceeds 5239 times that of non-catalytic systems. The newly designed small molecule organic complex effectively binds to and catalyzes the hydrolysis of substrate PNPA in accordance with Michaelis-Menten kinetics. The mechanism with activation by single Lewis acid provides fundamental insights for the development of small molecule hydrolases.

SUBMITTER: Li W 

PROVIDER: S-EPMC11914156 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature