Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells.

ABSTRACT: Spermatogenesis is an intricate and tightly controlled process encompassing various layers of gene expression regulation. Despite the advance of our current understanding, the developmental trajectory and regulatory mechanisms dictating spermatogenesis remain elusive. In this study, we have generated single-cell gene expression profiles for Caenorhabditis elegans sperm cells and constructed gene regulatory networks alongside the developmental trajectories of these cells. Our findings indicate that each pre- and post-developmental stage is closely linked by co-expressed genes, while simultaneously being uniquely identified by the combined expression of specific gene families. To illustrate the applicability of this exhaustive gene expression catalog, we used gene regulatory networks to unco

SUBMITTER: Li L 

PROVIDER: S-EPMC11914268 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature