<i>Ex vivo</i> removal of pro-fibrotic collagen and rescue of metabolic function in human ovarian fibrosis.

ABSTRACT: Tissue fibrosis, with the excessive accumulation of extracellular matrix, leads to organ dysfunction. The ovary shows signs of fibrosis from an early age, creating a permissive environment for ovarian cancer. A robust culture-platform to study human ovarian fibrosis would enable screens for antifibrotic drugs to prevent or even reverse this process. Based on previous results showing that androgen therapy can induce ovarian fibrosis, we characterized the fibrotic state of ovaries from transmasculine donors of reproductive age. Anti-inflammatory and antioxidant drugs, such as Pirfenidone, Metformin, and Mitoquinone, could reduce and revert the excess collagen content of the ovarian cortical tissue during culture. We demonstrated that Metformin exerts an antioxidant role and prevents a glycol

SUBMITTER: Del Valle JS 

PROVIDER: S-EPMC11914289 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature