Smooth muscle cell Piezo1 depletion results in impaired contractile properties in murine small bowel.

ABSTRACT: Piezo1 is a mechanosensitive cation channel expressed in intestinal muscularis cells (IMCs), including smooth muscle cells (SMCs), interstitial cells of Cajal, and Pdgfrα+ cells, which form the SIP syncytium, crucial for GI contractility. Here, we investigate the effects of SMC-specific Piezo1 deletion on small bowel function. Piezo1 depletion results in weight loss, delayed GI transit, muscularis thinning, and decreased SMCs. Ex vivo analyses demonstrated impaired contractile strength and tone, while in vitro studies using IMC co-cultures show dysrhythmic Ca²⁺ flux with decreased frequency. Imaging reveal that Piezo1 localizes intracellularly, thereby likely impacting Ca²⁺ signaling mechanisms modulated by Ca^2 + -handling channels located on the sarcoplasm

SUBMITTER: Bautista GM 

PROVIDER: S-EPMC11914552 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature