Identification and virulence factors prediction of Didymella segeticola causing leaf spot disease in Asarum heterotropoides in China.

ABSTRACT: Chinese wild ginger (Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag) is a perennial medicinal herb in China. In May 2022, 25 leaf-spot Asarum plants were collected. Employing tissue-separation and single-spore-isolation techniques, 37 fungal strains were obtained, of which seven were Didymella-like strains. Through multi-locus phylogeny and morphology analyses, these seven strains were identified as Didymella segeticola, with stain Xy0531-4 designated as the representative. In multi-locus phylogeny analysis, the concatenated fragments in the series were 1422 bp, including 395 bp of ITS, 532 bp of LSU, 358 bp of rpb2, and 137 bp of tub2. Pathogenicity tests confirmed that D. segeticola is the causal agent of Asarum leaf spot in China. Furthermore, through bioinformatic

SUBMITTER: Liu K 

PROVIDER: S-EPMC11914631 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature