Transcriptional heterogeneity shapes stress-adaptive responses in yeast.

ABSTRACT: In response to stress, cells activate signaling pathways that coordinate broad changes in gene expression to enhance cell survival. Remarkably, complex variations in gene expression occur even in isogenic populations and in response to similar signaling inputs. However, the molecular mechanisms underlying this variability and their influence on adaptive cell fate decisions are not fully understood. Here, we use scRNA-seq to longitudinally assess transcriptional dynamics during osmoadaptation in yeast. Our findings reveal highly heterogeneous expression of the osmoresponsive program, which organizes into combinatorial patterns that generate distinct cellular programs. The induction of these programs is favored by global transcriptome repression upon stress. Cells displaying basal expression

SUBMITTER: Nadal-Ribelles M 

PROVIDER: S-EPMC11914649 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature