Organelle-tuning condition robustly fabricates energetic mitochondria for cartilage regeneration.

ABSTRACT: Mitochondria are vital organelles whose impairment leads to numerous metabolic disorders. Mitochondrial transplantation serves as a promising clinical therapy. However, its widespread application is hindered by the limited availability of healthy mitochondria, with the dose required reaching up to 10⁹ mitochondria per injection/patient. This necessitates sustainable and tractable approaches for producing high-quality human mitochondria. In this study, we demonstrated a highly efficient mitochondria-producing strategy by manipulating mitobiogenesis and tuning organelle balance in human mesenchymal stem cells (MSCs). Utilizing an optimized culture medium (mito-condition) developed from our established formula, we achieved an 854-fold increase in mitochondria production compared to

SUBMITTER: Chen X 

PROVIDER: S-EPMC11914688 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature