Islet single-cell transcriptomic profiling during obesity-induced beta cell expansion in female mice.

ABSTRACT: Targeting beta cell proliferation is an appealing approach to restore glucose control in type 1 diabetes. However, the underlying mechanisms of beta cell proliferation remain incompletely understood, limiting identification of new therapeutic targets. Obesity is a naturally occurring process that potently induces human and rodent beta cell replication, representing an ideal model to study mechanisms of beta cell proliferation. We showed previously acute whole-body Lepr gene deletion in adult mice induces obesity and massive beta cell expansion. Here, using single-cell transcriptomics with female Lepr KO islets, we identified distinct populations of beta cells undergoing unfolded protein response (UPR), stress resolution, and cell cycle progression. Lepr KO beta cells u

SUBMITTER: Masschelin PM 

PROVIDER: S-EPMC11914824 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature