Advantages and Limitations of Photoconvertible Probes to Study Subcellular Dynamics in Epithelial Cells.

ABSTRACT: The recent development of a wide variety of genetically encoded photoconvertible fluorescent proteins has made it possible to study unprecedented dynamic processes by monitoring sub-populations of cells or labeled proteins. The use of photoconvertible fluorescent proteins, such as Eos, KAEDE, mMaple3, Dendra2 is a major advance. However, the conditions of their use in vivo and the inherent potential side-effects remain poorly characterized. Here, we used Drosophila pupal notum to characterize in vivo the conditions for photoconversion (PC) at the subcellular level. We compared the ability to photoconvert proteins exhibiting distinct localization and dynamics, namely, cytosolic and transmembrane proteins fused to photoconvertible probes and expressed at physiological levels. We report that

SUBMITTER: Pinot M 

PROVIDER: S-EPMC11914868 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature