Chromatin changes associated with neutrophil extracellular trap formation in whole blood reflect complex immune signaling.

ABSTRACT:

Background

Neutrophils are key players in innate immunity, forming neutrophil extracellular traps (NETs) to defend against infections. However, excess NET formation is implicated in inflammatory conditions such as sepsis and immunothrombosis. Studying NET formation in isolated neutrophils provides important mechanistic insights but does not reflect the complexity of immune interactions in whole blood, limiting our understanding of neutrophil responses.

Methods

This study investigates chromatin accessibility changes using Assay for Transposase-Accessible Chromatin with sequencing (ATAC-Seq) during phorbol 12-myristate 13-acetate (PMA) induced NET formation in whole blood. We compared chromatin accessibility patterns in neutrophils following PMA treatment in isolation and whol

SUBMITTER: Cayford J 

PROVIDER: S-EPMC11914908 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature