Printing Cell Embedded Sacrificial Strategy for Microvasculature using Degradable DNA Biolubricant.

ABSTRACT: Microvasculature is essential for the continued function of cells in tissue and is fundamental in the fields of tissue engineering, organ repair and drug screening. However, the fabrication of microvasculature is still challenging using existing strategies. Here, we developed a general PRINting Cell Embedded Sacrificial Strategy (PRINCESS) and successfully fabricated microvasculatures using degradable DNA biolubricant. This is the first demonstration of direct cell printing to fabricate microvasculature, which eliminates the need for a subsequent cell seeding process and the associated deficiencies. Utilizing the shear-thinning property of DNA hydrogels as a novel sacrificial, cell-laden biolubricant, we can print a 70 μm endothelialized microvasculature, breaking the limit of 100 μm. To o

SUBMITTER: Shi J 

PROVIDER: S-EPMC11914955 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature