A Novel Adductomics Workflow Incorporating <i>FeatureHunter</i> Software: Rapid Detection of Nucleic Acid Modifications for Studying the Exposome.

ABSTRACT: Exposure to the physicochemical agents that interact with nucleic acids (NA) may lead to modification of DNA and RNA (i.e., NA modifications), which have been associated with various diseases, including cancer. The emerging field of NA adductomics aims to identify both known and unknown NA modifications, some of which may also be associated with proteins. One of the main challenges for adductomics is the processing of massive and complex data generated by high-resolution tandem mass spectrometry (HR-MS/MS). To address this, we have developed a software called "FeatureHunter", which provides the automated extraction, annotation, and classification of different types of key NA modifications based on the MS and MS/MS spectra acquired by HR-MS/MS, using a user-defined feature list. The

SUBMITTER: Hu CW 

PROVIDER: S-EPMC11915021 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature