Iron-sulfur cluster proteins present the weak spot in non-thermal plasma-treated Escherichia coli.

ABSTRACT: Non-thermal atmospheric pressure plasmas have an antiseptic activity beneficial in different medical applications. In a genome-wide screening, hydrogen peroxide and superoxide were identified as key species contributing to the antibacterial effects of plasma while [FeS] cluster proteins emerged as potential cellular targets. We investigated the impact of plasma treatment on [FeS] cluster homeostasis in Escherichia coli treated for 1 min with the effluent of a microscale atmospheric pressure plasma jet (μAPPJ). Mutants defective in [FeS] cluster synthesis and maintenance lacking the SufBC₂D scaffold protein complex or desulfurase IscS were hypersensitive to plasma treatment. Monitoring the activity of [FeS] cluster proteins of the tricarboxylic acid cycle (aconitase, fumarase, su

SUBMITTER: Krewing M 

PROVIDER: S-EPMC11915174 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature