Sequential metabolic probes illuminate nuclear DNA for discrimination of cancerous and normal cells.

ABSTRACT: Elucidating the timing and spatial distribution of DNA synthesis within cancer cells is vital for cancer diagnosis and targeted therapy. However, current probes for staining nucleic acids rely on electrostatic interactions and hydrogen bonds with the nucleic acid, resulting in "static" DNA staining and the inability to distinguish cell types. Here, we present a proof-of-concept study of sequential metabolic probes, for the first time allowing for cancer-cell-specific illumination of DNA. This breakthrough is achieved by the combination of a "dual-locked" nucleoside analog VdU-Lys, and a new tetrazine-based bioorthogonal probe. Specifically, 5-vinyl-2'-deoxyuridine (VdU) release is only conducted in programmatically triggered histone deacetylases (HDACs) and cathepsin L (CTSL) as "sequentia

SUBMITTER: Liu C 

PROVIDER: S-EPMC11915456 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature