Expansion of the tmRNA sequence database and new tools for search and visualization.

ABSTRACT: Transfer-messenger RNA (tmRNA) contributes essential tRNA-like and mRNA-like functions during the process of trans-translation, a mechanism of quality control for the translating bacterial ribosome. Proper tmRNA identification benefits the study of trans-translation and also the study of genomic islands, which frequently use the tmRNA gene as an integration site. Automated tmRNA gene identification tools are available, but manual inspection is still important for eliminating false positives. We have increased our database of precisely mapped tmRNA sequences over 50-fold to 97 179 unique sequences. Group I introns had previously been found integrated within a single subsite within the TψC-loop; they have now been identified at four distinct subsites, suggesting multiple foundi

SUBMITTER: Nawrocki EP 

PROVIDER: S-EPMC11915505 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature