Solid-state NMR protocols for unveiling dynamics and (drug) interactions of membrane-bound proteins.

ABSTRACT: Magic angle spinning solid-state NMR (MAS ssNMR) is a versatile tool for studying the structure and dynamics of membrane proteins, as well as their interactions with ligands and drugs. Its power lies in the ability to provide atomic-level information on samples under physiological-like conditions. Moreover, it can illuminate dynamics across a wide range of timescales with great relevance to membrane protein function and dysfunction. In this protocol paper, we highlight key aspects of sample preparation, data acquisition, and interpretation, based on our own experience and the broader literature. We discuss key protocol steps along with important considerations for sample preparation and parameters for ssNMR measurements, with reference to the special requirements of membrane-based samples.

SUBMITTER: Lasorsa A 

PROVIDER: S-EPMC11915643 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature