Single-cell delineation of strain-specific HIV-1 Vif activities using dual reporter sensor cells and live cell imaging.

ABSTRACT: Human immunodeficiency virus type 1 (HIV-1) genome diversification is a key determinant of viral evolution and the pathogenesis of HIV/AIDS. Antiretroviral therapy is non-curative, and in the context of monitoring the latent reservoir, precision tools are needed to detect and enumerate HIV-1 genomes as well as to assess their heterogeneity, replication potential, and predict responses to therapy. Current sequencing-based methodologies are often unable to confirm intact genomes and most cell-based reporters provide limited information pertaining to viral fitness. In this study, we describe dual reporter sensor cells (DRSCs), an imaging-based reporter system designed to detect HIV-1 infection and measure several independent attributes of the virus in a single-cell high-content assay. We show

SUBMITTER: Guerrero JF 

PROVIDER: S-EPMC11915839 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature