Plasmid-borne <i>mcr-1</i> and replicative transposition of episomal and chromosomal <i>bla</i>_NDM-1, <i>bla</i>_OXA-69, and <i>bla</i>_OXA-23 carbapenemases in a clinical <i>Acinetobacter baumannii</i> isolate.

ABSTRACT: A multidrug-resistant clinical Acinetobacter baumannii isolate with resistance to most antibiotics was isolated from a patient at an intensive care unit. The genetic environment, transcriptome, mobile, and resistome were characterized. The MicroScan system, disc diffusion, and broth microdilution were used to determine the resistance profile of the isolate. A multiplex PCR assay was also used to screen for carbapenemases and mcr-1 to -5 resistance genes. Efflux-pump inhibitors were used to evaluate efflux activity. The resistome, mobilome, epigenome, and transcriptome were characterized. There was phenotypic resistance to 22 of the 25 antibiotics tested, intermediate resistance to levofloxacin and nalidixic acid, and susceptibility to tigecycline, which corresponded to the 27

SUBMITTER: Mmatli M 

PROVIDER: S-EPMC11915867 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature