Perturbation of <i>de novo</i> lipogenesis hinders MERS-CoV assembly and release, but not the biogenesis of viral replication organelles.

ABSTRACT: Coronaviruses hijack host cell metabolic pathways and resources to support their replication. They induce extensive host endomembrane remodeling to generate viral replication organelles and exploit host membranes for assembly and budding of their enveloped progeny virions. Because of the overall significance of host membranes, we sought to gain insight into the role of host factors involved in lipid metabolism in cells infected with Middle East respiratory syndrome coronavirus (MERS-CoV). We employed a single-cycle infection approach in combination with pharmacological inhibitors, biochemical assays, lipidomics, and light and electron microscopy. Pharmacological inhibition of acetyl-CoA carboxylase (ACC) and fatty acid synthase (FASN), key host factors in de novo fatty acid biosynth

SUBMITTER: Soultsioti M 

PROVIDER: S-EPMC11915874 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature