Improvement of Nanopore sequencing provides access to high quality genomic data for multi-component CRESS-DNA plant viruses.

ABSTRACT:

Background

Faced with the recrudescence of viral CRESS-DNA plant diseases, the availability of efficient and cost-effective tools for routine diagnosis and genomic characterisation is vital. As these viruses possess circular single-strand DNA genomes, they have been routinely characterised using rolling circle amplification (RCA) coupled with Sanger sequencing. However, while providing the basis of our knowledge of the diverse CRESS-DNA viruses, this approach is laboratory-intensive, time-consuming and ultimately ineffective faced with co-infection or viruses with multiple genomic components, two common characteristics of these viruses. Whereas alternatives have proved effective in some applications, there is a strong need for next-generation sequencing methods suitable for small-s

SUBMITTER: Otron DH 

PROVIDER: S-EPMC11917030 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature