Simvastatin modulates osteogenic differentiation in Stem Cells isolated from Apical Papilla.

ABSTRACT:

Background

Simvastatin modulates numerous stem cell functions, including stemness maintenance and differentiation. The present study aimed to explore the effect of simvastatin on the osteogenic differentiation of Stem Cells isolated from Apical Papilla (SCAPs) in vitro.

Methods

Cells were isolated from apical papilla, and mesenchymal stem cell features were characterised. Cells were treated with various concentrations of simvastatin (100-1,000 nM). The mRNA expression profile of simvastatin-treated SCAPs was examined using RNA sequencing technique. The osteogenic differentiation abilities were assessed. Alkaline phosphatase activity was determined. The mineralisation was visualised using Alizarin Red S and Von Kossa staining. The osteogenic marker gene expression was determi

SUBMITTER: Rewthamrongsris P 

PROVIDER: S-EPMC11917056 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature