The effects of free fatty acid-free bovine serum albumin and palmitate on pancreatic β-cell function.

ABSTRACT: Pancreatic β-cells release insulin in response to fluctuations in plasma glucose, amino acids, and free fatty acids (FFA). Clonal cell lines and isolated islets serve as essential early models for studying the impact of nutrients and evaluating potential therapies to address β-cell dysfunction. Acute and chronic changes in FFA levels have been shown to have positive and negative effects on β-cell function both in vivo and in vitro. A key problem in comparing islet lipid studies from different laboratories is that a wide variety of methods are used to isolate, culture, and assess islet function. The current study compares bovine serum albumin (BSA) types and lipid preparation methods in clonal 832/13 cells and human islets. Changing the percentage and culture conditions when u

SUBMITTER: Sentjens K 

PROVIDER: S-EPMC11917175 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature