In vivo identification of astrocyte and neuron subproteomes by proximity-dependent biotinylation.

ABSTRACT: The central nervous system (CNS) comprises diverse and morphologically complex cells. To understand the molecular basis of their physiology, it is crucial to assess proteins expressed within intact cells. Commonly used methods utilize cell dissociation and sorting to isolate specific cell types such as neurons and astrocytes, the major CNS cells. Proteins purified from isolated cells are identified by mass spectrometry-based proteomics. However, dissociation and cell-sorting methods lead to near total loss of cellular morphology, thereby losing proteins from key relevant subcompartments such as processes, end feet, dendrites and axons. Here we provide a systematic protocol for cell- and subcompartment-specific labeling and identification of proteins found within intact astrocytes and neuro

SUBMITTER: Soto JS 

PROVIDER: S-EPMC11917372 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature