Liquid chromatograph-mass spectrometry metabolomics uncovers potential biomarkers of semen cryo-injury in goats.

ABSTRACT:

Objective

Semen cryopreservation acts a crucial role in enhancing breed improvement and conserving genetic resources. However, it often leads to decreased sperm activity and reduced pregnancy rates. Despite significant advancements in semen freezing techniques for goats, the precise factors and mechanisms causing cryo-injury remain unclear.

Methods

In this study, we examined the motility characteristics of fresh semen versus frozen-thawed semen and investigated changes in the metabolite profiles of seminal plasma using liquid chromatograph-mass spectrometry.

Results

A total of 364 differentially expressed metabolites (DEMs) were identified between fresh and frozen-thawed semen samples. Among these, 185 metabolites were significantly up-regulated, while 179 were down-r

SUBMITTER: Wu S 

PROVIDER: S-EPMC11917422 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature